Application
Immunofluorescence of NKX-8 in human liver tissue with NKX2-8 antibody at 20 μg/mL.Application
Immunohistochemistry of NKX2-8 in human liver tissue with NKX2-8 antibody at 2.5 μg/mL.Application
Western blot analysis of NKX2-8 in rat liver tissue lysate with NKX2-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | NKX2-8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NKX2-8 Antibody: NKX2H, NKX2.8, Nkx2-9, NKX-2.8, NKX2G, NKX2H, Homeobox protein NK-2 homolog H |
| Product description | NKX2-8 Antibody: NKX2-8 (NK2 homeobox 8) is a member of a family of transcription factors that are involved in embryonic development and cell fate. It is expressed in the ventral foregut, the developing heart, the epithelial layers of the branchial arches and in the dorsal mesoderm. In conjunction with related protein, NKX2-5, NKX2-8 may play a role in cardiac embryonic development. NKX2-8 is also thought to be involved in lung development and is suspected of being an oncogene in lung cancer that is activated by way of gene amplification at chromosome 14q13.1) Boettger T, Stein S, and Kessel M. The chicken NKX2.8 homeobox gene: A novel member of the NK-2 gene family. Dev. Genes Evol. 1997; 207:65-70. |
| Immunogen | NKX2-8 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-8. The immunogen is located within amino acids 60 - 110 of NKX2-8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NKX2-8 antibody can be used for detection of NKX2-8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKX2-8 |
|---|---|
| Alternative Names | NKX2-8 Antibody: NKX2H NKX2.8 Nkx2-9 NKX-2.8 NKX2G NKX2H Homeobox protein NK-2 homolog H |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | NKX2-8 antibody is predicted to not cross-react with other NK2 homeobox family members. |
Application
Immunofluorescence of NKX-8 in human liver tissue with NKX2-8 antibody at 20 μg/mL.
Application
Immunohistochemistry of NKX2-8 in human liver tissue with NKX2-8 antibody at 2.5 μg/mL.
Application
Western blot analysis of NKX2-8 in rat liver tissue lysate with NKX2-8 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | NKX2-8 antibody can be used for detection of NKX2-8 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NKX2-8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NKX2-8 Antibody is supplied in PBS containing 0.02% sodium azide. |
