Application
Immunohistochemistry of NLGN2 in mouse brain tissue with NLGN2 antibody at 5 μg/ml.Application
Immunofluorescence of NLGN2 in mouse brain tissue with NLGN2 antibody at 20 μg/ml.Application
Western blot analysis of NLGN2 in rat brain tissue lysate with NLGN2 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | NLGN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuroligin 2 |
| Product description | Neuroligin 2 (NLGN2) is a member of a family of neuronal cell surface proteins that localize to the post-synaptic membrane (1,2). Neuroligins are thought to act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses (2). NLGN2 has been shown to control perisomatic inhibitory synapse maturation together with gephyrin and collybistin, thereby regulating GABA receptor clustering on neurons (3,4). NLGN2 also exhibits differential functions at different types of inhibitory synapses on the same postsynaptic neuron (5).1) Ichtchenko K, Nguyen T, and Sudhof TC. Structures, alternative splicing, and neurexin binding of multiple neuroligins. J. Biol. Chem. 1996; 271:2676-82. |
| Immunogen | NLGN2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human NLGN2. The immunogen is located within amino acids 630 - 680 of NLGN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF |
| NLGN2 antibody can be used for detection of NLGN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLGN2 |
|---|---|
| Alternative Names | Neuroligin 2 |
| Molecular Weight(MW) | Predicted: 92, 99 kDa Observed: 80, 97 kDa |
| Tissue Specificity | NLGN2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NLGN2 antibody is predicted to not cross-react with other members of the NLGN protein family. |
Application
Immunohistochemistry of NLGN2 in mouse brain tissue with NLGN2 antibody at 5 μg/ml.
Application
Immunofluorescence of NLGN2 in mouse brain tissue with NLGN2 antibody at 20 μg/ml.
Application
Western blot analysis of NLGN2 in rat brain tissue lysate with NLGN2 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | NLGN2 antibody can be used for detection of NLGN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NLGN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NLGN2 antibody is supplied in PBS containing 0.02% sodium azide. |
