Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 N| Product Name | NMDA (phospho Tyr1252) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GluN2B, N-methyl D-aspartate receptor subtype 2B, GluN2B |
| Product description | The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1252 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002).1) Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1252 of the NR2B subunit of the rat NMDA receptor. |
| Modification | p-Tyr1252 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | BovineDogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Grin2b |
|---|---|
| Alternative Names | GluN2B N-methyl D-aspartate receptor subtype 2B GluN2B |
| Molecular Weight(MW) | 180 |
Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 N| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NMDA antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
