Application
Western blot of rat hippocampal lysate showing phosphospecific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472.| Product Name | NMDA NR2B (phospho Tyr1472) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GluN2B, N-methyl D-aspartate receptor subtype 2B, GluN2B |
| Product description | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.1) Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications," Pharmacol. Ther., 97 (2003) 55 - 85. |
| Immunogen | NMDA NR2B (Tyr1472) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Tyr1472 of rat NMDA NR2B. |
| Modification | p-Tyr1472 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The labeling of the NR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at 65k and 115k. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Grin2b |
|---|---|
| Alternative Names | GluN2B N-methyl D-aspartate receptor subtype 2B GluN2B |
| Molecular Weight(MW) | 180 |
| Tissue Specificity | NMDA antibody is specific for ~180 kD NMDAR NR2B phosphorylated at Tyr1472. |
Application
Western blot of rat hippocampal lysate showing phosphospecific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472.| Application Notes | The labeling of the NR2B protein in Western blots of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at 65k and 115k. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
