Application
Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.| Product Name | NMDA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR2A, GluN2A, NMDAR2A, N-methyl D-aspartate receptor subtype 2A |
| Product description | The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.1) Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. |
| Immunogen | Fusion protein from the C-terminus region of the NR2A subunit of rat NMDA receptor. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Serum |
| Gene Symbol | Grin2a |
|---|---|
| Alternative Names | NR2A GluN2A NMDAR2A N-methyl D-aspartate receptor subtype 2A |
| Molecular Weight(MW) | 180 |
Application
Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.| Application Notes | The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | NMDA antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
