Application
NMDA NR2A Subunit Antibody| Product Name | NMDA NR2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR2A, GluN2A, NMDAR2A, N-methyl D-aspartate receptor subtype 2A |
| Product description | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR2A subunit of the receptor has been associated synaptic plasticity.1) Loftis, J.M. and Janowsky, A., "The N-methyl-D-aspartate receptor subunit NR2B: localization, functional properties, regulation, and clinical implications," Pharmacol. Ther., 97 (2003) 55 - 85. |
| Immunogen | NMDA NR2A polyclonal antibody was raised against a C-terminal fusion protein of the NR2A subunit of the NMDAR. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| NMDA NR2A Subunit antibody is specific for the 180k NR2A subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2A subunit that was used to generate the antibody. No reactivity towards the NR2B subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB, and 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2A for IP.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Grin2a |
|---|---|
| Alternative Names | NR2A GluN2A NMDAR2A N-methyl D-aspartate receptor subtype 2A |
| Molecular Weight(MW) | 180 |
Application
NMDA NR2A Subunit Antibody| Application Notes | NMDA NR2A Subunit antibody is specific for the 180k NR2A subunit in Western blots of most brain regions. Immunolabeling blocked by pre-adsorption of antibody with the C-terminal fusion protein of the NR2A subunit that was used to generate the antibody. No reactivity towards the NR2B subunit of the NMDAR. Applications include Dot Blots (DB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blots (WB). Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB, and 3 μg/200 μg lysate will quantitatively precipitate all NMDAR 2A for IP.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NMDA NR2A Subunit antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C and is stable at -20˚C for at least 1 year. Ambient shipment is suitable for both international and domestic. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
