Application
NMDA NR1 Subunit Antibody| Product Name | NMDA NR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR1, GluN1, NMDAR1, Nmdar1, N-methyl-D-aspartate receptor subunit NR1 |
| Product description | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s disease, epilepsy and ischemic neuronal cell death. Increased membrane surface expression of the NR1 subunit of the receptor has been associated with synaptic plasticity.1) Luo, J.H., Wang, Y.H., Yasuda, R.P., Dunah, A.W. and Wolfe, B.B., "The majority of N-methyl-D-aspartate receptor complexes in adult rat cerebral cortex contain at least three different subunits (NR1/NR2A/NR2B)," Mol. Pharmacol. 51 (1997) 79 - 86. |
| Immunogen | NMDA NR1 Subunit monoclonal antibody was raised against a fusion protein from AA 1 - 564 of NMDA NR1. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| NMDA NR1 Subunit antibody allows for strong and specific immunolabeling of the 120k NR1 protein in Western blots of rat brain hippocampal homogenate. Applications include Dot Blots (DB), Immunoprecipitation (IP) and Western Blots (WB). Mouse anti-NMDA NR1 Subunit recognizes NR1 in human, mouse and rat brain lysates. When internally tested under ideal conditions the working dilutions were 1:300 for DB and WB, and 3 μg per 200 μg lysate (will quantitatively precipitate all the NMDA NR1 in 200 μg of rat brain lysate).: |
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| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Grin1 |
|---|---|
| Alternative Names | NR1 GluN1 NMDAR1 Nmdar1 N-methyl-D-aspartate receptor subunit NR1 |
| Molecular Weight(MW) | 120 |
Application
NMDA NR1 Subunit Antibody| Application Notes | NMDA NR1 Subunit antibody allows for strong and specific immunolabeling of the 120k NR1 protein in Western blots of rat brain hippocampal homogenate. Applications include Dot Blots (DB), Immunoprecipitation (IP) and Western Blots (WB). Mouse anti-NMDA NR1 Subunit recognizes NR1 in human, mouse and rat brain lysates. When internally tested under ideal conditions the working dilutions were 1:300 for DB and WB, and 3 μg per 200 μg lysate (will quantitatively precipitate all the NMDA NR1 in 200 μg of rat brain lysate).: |
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| Form | Liquid |
|---|---|
| Storage Instructions | NMDA NR1 Subunit antibody can be stored at -20˚C. After reconstitution in 50 μl water, the antibody should be aliquot and stored at -20˚C. This product is stable at -20˚C for at least 1 year and can be shipped ambient domestic or international. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
