Application
Immunofluorescence staining of methanol-fixed A549 cells using NMDAR1 (Phospho-Ser890) Antibody.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using NMDAR1 (Phospho-Ser890) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | NMDAR1 (phospho Ser890) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLURZ1, GRIN1, NMD-R1, NMDZ1, NMZ1 |
| Product description | NMDA receptors are members of the ionotropic class of glutamate receptors, which also includes Kainate and AMPA receptors. NMDA receptors consist of NR1 subunits combined with one or more NR2 (A-D) or NR3 (A-B) subunits. The ligand-gated channel is permeable to cations including Ca2+, and at resting membrane potentials NMDA receptors are inactive due to a voltage-dependent blockade of the channel pore by Mg2+. NMDA receptor activation, which requires binding of glutamate and glycine, leads to an influx of Ca2+ into the postsynaptic region where it activates several signaling cascades, including pathways leading to the induction of long-term potentiation (LTP) and depression (LTD). NMDA receptors have a critical role in excitatory synaptic transmission and plasticity in the CNS. They govern a range of physiological conditions including neurological disorders caused by excitotoxic neuronal injury, psychiatric disorders and neuropathic pain syndromes.1) Guang Bai, J. Biol. Chem., Jan 1998; 273: 1086. |
| Immunogen | NMDAR1 (phospho Ser890) antibody was raised against a peptide sequence around phosphorylation site of Serine 890 (A-S-S (p)-F-K) derived from Human NMDAR1. |
| Modification | p-Ser890 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRIN1 |
|---|---|
| Alternative Names | GLURZ1 GRIN1 NMD-R1 NMDZ1 NMZ1 |
| Molecular Weight(MW) | 120 kDa |
| Tissue Specificity | The antibody detects endogenous levels of NMDAR1 only when phosphorylated at serine 890. |
| Entrez Gene | 2902 |
|---|---|
| Protein Accession | NP_015566.1 |
Application
Immunofluorescence staining of methanol-fixed A549 cells using NMDAR1 (Phospho-Ser890) Antibody.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using NMDAR1 (Phospho-Ser890) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
