Application
Antibody used in WB on Human HepG2 at 0.25 ug/ml.| Product Name | NMNAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NMNAT1, NMNAT, PNAT-1, PNAT1, LCA9 |
| Product description | The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca (2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca (2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]).1) Sorci, L., (2007) Biochemistry 46 (16), 4912-4922. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NMNAT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NMNAT1 antibody can be used for detection of NMNAT1 by ELISA at 1:12500. NMNAT1 antibody can be used for detection of NMNAT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NMNAT1 |
|---|---|
| Alternative Names | NMNAT1 NMNAT PNAT-1 PNAT1 LCA9 |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Human HepG2 at 0.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NMNAT1 antibody can be used for detection of NMNAT1 by ELISA at 1:12500. NMNAT1 antibody can be used for detection of NMNAT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NMNAT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
