Application
Western Blot at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.| Product Name | NMNAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nicotinamide mononucleotide adenylyltransferase 1, NMN adenylyltransferase 1, 2.7.7.1, Nicotinate-nucleotide adenylyltransferase 1, NaMN adenylyltransferase 1, 2.7.7.18, NMNAT1, NMNAT |
| Product description | Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NAAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following mechanical or toxic insults.1) Schweiger M.,et al.FEBS Lett. 492:95-100(2001). |
| Immunogen | This NMNAT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 200-232 amino acids from human NMNAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NMNAT1 |
|---|---|
| Alternative Names | Nicotinamide mononucleotide adenylyltransferase 1 NMN adenylyltransferase 1 2.7.7.1 Nicotinate-nucleotide adenylyltransferase 1 NaMN adenylyltransferase 1 2.7.7.18 NMNAT1 NMNAT |
| Molecular Weight(MW) | 32 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
