| Product Name | NNT-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLC, NR6, BSF3, NNT1, BSF-3, CISS2, NNT-1, CLC, Cardiotrophin-like cytokine factor 1, B-cell-stimulating factor 3 |
| Product description | NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNNT-1/BCSF-3. Human NNT-1/BCSF-3 specific antibody was purified by affinity chromatography employing immobilized hNNT-1/BCSF-3 matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Indirect: To detect hNNT-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1. Sandwich To detect hNNT-1by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NNT-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1. Western Blot: To detect hNNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CLCF1 |
|---|---|
| Alternative Names | CLC NR6 BSF3 NNT1 BSF-3 CISS2 NNT-1 CLC Cardiotrophin-like cytokine factor 1 B-cell-stimulating factor 3 |
| Application Notes | ELISA: Indirect: To detect hNNT-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1. Sandwich To detect hNNT-1by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human NNT-1 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNNT-1. Western Blot: To detect hNNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NNT-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
