Application
Immunocytochemistry of NOD2 in HeLa cells with NOD2 antibody at 10 μg/mL.Application
Immunofluorescence of NOD2 in HeLa cells with NOD2 antibody at 20 μg/mL.Application
Western blot analysis of NOD2 in HeLa cell lysate with NOD2 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | NOD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOD2 Antibody: CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1, Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15 |
| Product description | NOD2 Antibody: Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease that is a main type of chronic inflammatory bowel disease.1) Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-κB. J. Biol. Chem. 1999; 274:14560-7. |
| Immunogen | Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 14 amino acids at the carboxy terminus of human NOD2. The immunogen is located within the last 50 amino acids of NOD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOD2 |
|---|---|
| Alternative Names | NOD2 Antibody: CD ACUG BLAU IBD1 NLRC2 NOD2B CARD15 CLR16.3 PSORAS1 Nucleotide-binding oligomerization domain-containing protein 2 Caspase recruitment domain-containing protein 15 |
| Molecular Weight(MW) | 114 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | NOD2 has no cross-reaction with NOD1. |
Application
Immunocytochemistry of NOD2 in HeLa cells with NOD2 antibody at 10 μg/mL.
Application
Immunofluorescence of NOD2 in HeLa cells with NOD2 antibody at 20 μg/mL.
Application
Western blot analysis of NOD2 in HeLa cell lysate with NOD2 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | NOD2 antibody can be used for detection of Noxa by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NOD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
