WB
Western blot analysis of extracts of various cell lines, using NOD2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat An ti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 60s.IHC
Immunohistochemistry of paraffin-embedded human colon using NOD2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.ICC/IF
Immunofluorescence analysis of HeLa cells using NOD2 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.| Product Name | NOD2 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human NOD2(NP_071445.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOD2 |
|---|---|
| Gene Synonyms | CD ACUG BLAU IBD1 YAOS BLAUS NLRC2 NOD2B CARD15 CLR16.3 PSORAS1 |
| Gene Full Name | nucleotide binding oligomerization domain containing 2 |
| Gene Summary | This gene is a member of the Nod1/Apaf-1 family and encodes a protein with two caspase recruitment (CARD) domains and six leucine-rich repeats (LRRs). The protein is primarily expressed in the peripheral blood leukocytes. It plays a role in the immune response to intracellular bacterial lipopolysaccharides (LPS) by recognizing the muramyl dipeptide (MDP) derived from them and activating the NFKB protein. Mutations in this gene have been associated with Crohn disease and Blau syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| Molecular Weight(MW) | 100kDa |
| Cellular Localization | basolateral plasma membrane, cell surface, cytoplasm, cytoskeleton, cytosol, Golgi apparatus, mitochondrion, phagocytic vesicle, plasma membrane. |
WB
Western blot analysis of extracts of various cell lines, using NOD2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat An ti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 60s.
IHC
Immunohistochemistry of paraffin-embedded human colon using NOD2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Immunofluorescence analysis of HeLa cells using NOD2 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
