Application
Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.Application
Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NOD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOD3 Antibody: NOD3, CLR16.2, NOD3, Protein NLRC3, CARD15-like protein |
| Product description | NOD3 Antibody: NOD3 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD3 also known as NLR family CARD containing 3 (NLRC3), is predominantly expressed in the immune system, particularly in T lymphocytes, and its expression is strongly down-regulated following stimulation of the T-cell receptor complex and CD28, suggesting that NOD3 plays a role in attenuating the activation of T cells. NOD3 inhibits NF-kappaB, AP-1 and NFAT transcriptional activation in Jurkat T cells downstream of CD3/CD28 stimulation or treatment with PMA/ionomycin and decreases IL-2 and CD25 mRNA induction in activated cells.1) Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci.2006; 1072:19-27. |
| Immunogen | NOD3 antibody was raised against an 18 amino acid synthetic peptide near the center of human NOD3. The immunogen is located within amino acids 630 - 680 of NOD3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRC3 |
|---|---|
| Alternative Names | NOD3 Antibody: NOD3 CLR16.2 NOD3 Protein NLRC3 CARD15-like protein |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunofluorescence of NOD3 in Jurkat cells with NOD3 antibody at 20 μg/mL.
Application
Western blot analysis of NOD3 in 3T3 cell lysate with NOD3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | NOD3 antibody can be used for detection of NOD3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NOD3 Antibody is supplied in PBS containing 0.02% sodium azide. |
