Application
Western blot analysis of NOD5 in rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of NOD5 in rat spleen tissue with NOD5 antibody at 20 μg/mL.Application
Immunohistochemistry of NOD5 in rat spleen tissue with NOD5 antibody at 10 μg/mL.| Product Name | NOD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOD5 Antibody: NOD5, NOD9, NOD26, DLNB26, CLR11.3, NOD5, NLR family member X1, Nucleotide-binding oligomerization domain protein 26 |
| Product description | NOD5 Antibody: NOD5, also known as NLRX1, is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD5 localizes to the mitochondrial outer membrane and interacts with the virus-induced signaling adapter protein VISA. Unlike a subset of NOD-like receptors (NLRs) such as NOD1 and NOD2 which trigger pro-inflammatory cascades, and other NLRs that induce the caspase 1 inflammasome in response to immune challenges, NOD5 amplifies NF-κB and JNK pathways by inducing reactive oxygen species production.1) Kufer TA, Banks DJ, and Philpott DJ. Innate immune sensing of microbes by Nod proteins. Ann. NY Acad. Sci.2006; 1072:19-27. |
| Immunogen | NOD5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human NOD5. The immunogen is located within amino acids 70 - 120 of NOD5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NOD5 antibody can be used for detection of NOD5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRX1 |
|---|---|
| Alternative Names | NOD5 Antibody: NOD5 NOD9 NOD26 DLNB26 CLR11.3 NOD5 NLR family member X1 Nucleotide-binding oligomerization domain protein 26 |
Application
Western blot analysis of NOD5 in rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of NOD5 in rat spleen tissue with NOD5 antibody at 20 μg/mL.
Application
Immunohistochemistry of NOD5 in rat spleen tissue with NOD5 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | NOD5 antibody can be used for detection of NOD5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOD5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NOD5 Antibody is supplied in PBS containing 0.02% sodium azide. |
