Related to nucleologenesis, NOLC1 may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. NOLC1 may play an important role in transcription catalyzed by RNA polymerase I.1) Wang, C., (2002) Mol. Cell. Biol. 22 (24), 8457-8466.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOLC1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NOLC1 antibody can be used for detection of NOLC1 by ELISA at 1:62500. NOLC1 antibody can be used for detection of NOLC1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in IHC on Zebrafish at 0.004 ug/mL.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
NOLC1 antibody can be used for detection of NOLC1 by ELISA at 1:62500. NOLC1 antibody can be used for detection of NOLC1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOLC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt