Application
Western blot analysis of Norrin in Jurkat cell lysate with Norrin antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of Norrin in Jurkat cells with Norrin antibody at 5 μg/mL.| Product Name | Norrin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Norrin Antibody: ND, EVR2, FEVR, Norrin, Norrie disease protein |
| Product description | Norrin Antibody: Norrie disease is an X-linked genetic disorder characterized by progressive atrophy of the eyes, mental disturbances and deafness. The gene responsible for this disease was initially identified through positional cloning. Norrin, the gene product, encodes a small secreted, cysteine-rich protein that is thought to act as a ligand for the Wnt-receptor/beta-catenin signal pathway despite having sequence homology with the Wnt family of proteins. Mice lacking this gene have abnormal blood vessel growth in the vitreous and a disorganized retina; transgenic ectopic expression of Norrin restores normal retinal vasculature. Recent evidence shows that Norrin can attenuate tPA and uPA-mediated death of transformed rat retinal ganglion cells (RGC-5) by activating the Wnt/beta-catenin pathway and regulating the phosphorylation of LRP-1, a cell surface receptor for tPA and uPA, suggesting the Norrin may function in vivo by regulating kinases which may alter the phosphorylation of LRP-1.1) Bergen W, Meindl A, van de Pol TJ, et al. Isolation of a candidate gene for Norrie disease by positional cloning. Nat. Genet.1992; 1:199-203. |
| Immunogen | Norrin antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human Norrin. The immunogen is located within the first 50 amino acids of Norrin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Norrin antibody can be used for detection of Norrin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDP |
|---|---|
| Alternative Names | Norrin Antibody: ND EVR2 FEVR Norrin Norrie disease protein |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Western blot analysis of Norrin in Jurkat cell lysate with Norrin antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of Norrin in Jurkat cells with Norrin antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Norrin antibody can be used for detection of Norrin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Norrin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Norrin Antibody is supplied in PBS containing 0.02% sodium azide. |
