Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of lysate from MCF-7 cell line, using NOS1AP Antibody at 1:1000 at each lane.| Product Name | NOS1AP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, C-terminal PDZ ligand of neuronal nitric oxide synthase protein, Nitric oxide synthase 1 adaptor protein, NOS1AP, CAPON, KIAA0464 |
| Product description | This gene encodes a cytosolic protein that binds to the signaling molecule, neuronal nitric oxide synthase (nNOS). This protein has a C-terminal PDZ-binding domain that mediates interactions with nNOS and an N-terminal phosphotyrosine binding (PTB) domain that binds to the small monomeric G protein, Dexras1. Studies of the related mouse and rat proteins have shown that this protein functions as an adapter protein linking nNOS to specific targets, such as Dexras1 and the synapsins. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This NOS1AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-466 amino acids from the Central region of human NOS1AP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS1AP |
|---|---|
| Alternative Names | Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein C-terminal PDZ ligand of neuronal nitric oxide synthase protein Nitric oxide synthase 1 adaptor protein NOS1AP CAPON KIAA0464 |
| Molecular Weight(MW) | 56 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of lysate from MCF-7 cell line, using NOS1AP Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
