Application
Immunofluorescence of NOSTRIN in mouse kidney tissue with NOSTRIN antibody at 20 μg/ml.Application
Immunohistochemistry of NOSTRIN in mouse kidney tissue with NOSTRIN antibody at 5 μg/ml.| Product Name | NOSTRIN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nitric oxide synthase traffic inducer, eNOS-trafficking inducer, Nitric oxide synthase trafficker, BM247 homolog, DaIP2 |
| Product description | NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1,2). It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3). NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4,5).1) Zimmermann K, Opitz N, Dedio J, et al. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. USA 2002; 99:17167-72. |
| Immunogen | NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN. The immunogen is located within amino acids 430 - 480 of NOSTRIN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOSTRIN |
|---|---|
| Alternative Names | Nitric oxide synthase traffic inducer eNOS-trafficking inducer Nitric oxide synthase trafficker BM247 homolog DaIP2 |
| Molecular Weight(MW) | Predicted: 47, 53, 56, 62 kDa Observed: 68 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms. |
Application
Immunofluorescence of NOSTRIN in mouse kidney tissue with NOSTRIN antibody at 20 μg/ml.
Application
Immunohistochemistry of NOSTRIN in mouse kidney tissue with NOSTRIN antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1221 - SW480 Cell Lysate |
|---|---|
| Application Notes | NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOSTRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NOSTRIN antibody is supplied in PBS containing 0.02% sodium azide. |
