Application
Antibody used in WB on Human K562 at 0.2-1 ug/ml.| Product Name | NOTCH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOTCH3, IMF2, CASIL, CADASIL |
| Product description | NOTCH3 encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).1) Rufa, A., et al., (2004) Neurol. 61 (4), 577-580. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOTCH3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NOTCH3 antibody can be used for detection of NOTCH3 by ELISA at 1:12500. NOTCH3 antibody can be used for detection of NOTCH3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOTCH3 |
|---|---|
| Alternative Names | NOTCH3 IMF2 CASIL CADASIL |
| Molecular Weight(MW) | 244 kDa |
Application
Antibody used in WB on Human K562 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | NOTCH3 antibody can be used for detection of NOTCH3 by ELISA at 1:12500. NOTCH3 antibody can be used for detection of NOTCH3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOTCH3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
