Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Confocal immunofluorescent analysis of NOTCH3 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTdetected the NOTCH3 protein (arrow).| Product Name | NOTCH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neurogenic locus notch homolog protein 3, Notch 3, Notch 3 extracellular truncation, Notch 3 intracellular domain, NOTCH3 |
| Product description | NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).1) Suwanwela, N., et al.,Med Assoc Thai 86(2):178-182 (2003). Ahearn, E.P., et al., Am. J. Med. Genet. 114(6):652-658 (2002). Bellavia, D., et al., Proc. Natl. Acad. Sci. U.S.A. 99(6):3788-3793 (2002). Ito, D., et al., J. Neurol. Neurosurg. Psychiatr. 72(3):382-384 (2002). Joutel, A., et al., Lancet 350(9090):1511-1515 (1997). |
| Immunogen | This NOTCH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | NOTCH3 |
|---|---|
| Alternative Names | Neurogenic locus notch homolog protein 3 Notch 3 Notch 3 extracellular truncation Notch 3 intracellular domain NOTCH3 |
| Molecular Weight(MW) | 244 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Human |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Confocal immunofluorescent analysis of NOTCH3 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the NOTdetected the NOTCH3 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
