Application
Immunohistochemistry of NOX3 in human kidney tissue with NOX3 antibody at 5 μg/ml.Application
Immunofluorescence of NOX3 in human kidney tissue with NOX3 antibody at 20 μg/ml.Application
Western blot analysis of NOX3 in 293 cell lysate with NOX1 antibody at 1 μg/ml.| Product Name | NOX3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAPDH oxidase 3, NOX-3, GP91-3, MOX2, MOX-2 |
| Product description | The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith (2,3). It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss (4).1) Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313. |
| Immunogen | NOX3 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX3. The immunogen is located within amino acids 110 - 160 of NOX3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| NOX3 antibody can be used for detection of NOX3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOX3 |
|---|---|
| Alternative Names | NAPDH oxidase 3 NOX-3 GP91-3 MOX2 MOX-2 |
| Molecular Weight(MW) | Predicted: 62 kDa Observed: 72 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | NOX3 antibody is human and mouse reactive. NOX3 is predicted to not cross-react with other NOX proteins. |
Application
Immunohistochemistry of NOX3 in human kidney tissue with NOX3 antibody at 5 μg/ml.
Application
Immunofluorescence of NOX3 in human kidney tissue with NOX3 antibody at 20 μg/ml.
Application
Western blot analysis of NOX3 in 293 cell lysate with NOX1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | NOX3 antibody can be used for detection of NOX3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOX3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NOX3 antibody is supplied in PBS containing 0.02% sodium azide. |
