Application
Immunohistochemistry of NOXA in human stomach tissue with NOXA antibody at 2.5 μg/ml.Application
Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/mL.Application
Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 μg/mL.Application
Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | Noxa Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Noxa Antibody: Noxa, Phorbol-12-myristate-13-acetate-induced protein 1 |
| Product description | Noxa Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.1) Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8. |
| Immunogen | Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa. The immunogen is located within the first 50 amino acids of Noxa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Noxa antibody can be used for detection of Noxa by Western blot at at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Pmaip1 |
|---|---|
| Alternative Names | Noxa Antibody: Noxa Phorbol-12-myristate-13-acetate-induced protein 1 |
| Sequence Similarities | 2) Cat. No. 10-801 - Human Stomach Tissue Slide |
Application
Immunohistochemistry of NOXA in human stomach tissue with NOXA antibody at 2.5 μg/ml.
Application
Immunohistochemistry of Noxa in human stomach tissue with Noxa antibody at 1 μg/mL.
Application
Immunofluorescence of Noxa in Human Stomach cells with Noxa antibody at 10 μg/mL.
Application
Western blot analysis of Noxa in human stomach tissue lysate with Noxa antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1319 - Human Stomach Tissue Lysate |
|---|---|
| Application Notes | Noxa antibody can be used for detection of Noxa by Western blot at at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Noxa antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Noxa Antibody is supplied in PBS containing 0.02% sodium azide. |
