Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.Application
Antibody used in WB on Human THP-1 at 0.2-1 ug/ml.| Product Name | NP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NP, MGC117396, MGC125915, MGC125916, PNP, PRO1837, PUNP, NP |
| Product description | Defects in NP are the cause of nucleoside phosphorylase deficiency (NP deficiency). It leads to a severe T-cell immunodeficiency with neurologic disorder in children. The specific function of NP is not yet known.This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.1) Luo, M., (2008) Biochemistry 47 (8), 2565-2576 . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NP antibody can be used for detection of NP by ELISA at 1:62500. NP antibody can be used for detection of NP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PNP |
|---|---|
| Alternative Names | NP MGC117396 MGC125915 MGC125916 PNP PRO1837 PUNP NP |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Human THP-1 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1208 - THP-1 Cell Lysate |
|---|---|
| Application Notes | NP antibody can be used for detection of NP by ELISA at 1:62500. NP antibody can be used for detection of NP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NP antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM286441