Application
Western blot analysis of NPAS3 in SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of NPAS3 in human brain tissue with NPAS3 antibody at 20 μg/mL.Application
Immunohistochemistry of NPAS3 in human brain tissue with NPAS3 antibody at 5 μg/mL.| Product Name | NPAS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPAS3 Antibody: MOP6, PASD6, bHLHe12, BHLHE12, MOP6, Neuronal PAS domain-containing protein 3, Basic-helix-loop-helix-PAS protein MOP6, Neuronal PAS3 |
| Product description | NPAS3 Antibody: Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3-null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.1) Brunskill EW, Witte DP, Shreiner AB et al. Characterization of npas3, a novel basic helix-loop-helix PAS gene expressed in the developing mouse nervous system. Mech. Dev.1999; 88:237-41. |
| Immunogen | NPAS3 antibody was raised against a 28 amino acid synthetic peptide from near the amino terminus of human NPAS3. The immunogen is located within amino acids 40 - 90 of NPAS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NPAS3 antibody can be used for detection of NPAS3 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPAS3 |
|---|---|
| Alternative Names | NPAS3 Antibody: MOP6 PASD6 bHLHe12 BHLHE12 MOP6 Neuronal PAS domain-containing protein 3 Basic-helix-loop-helix-PAS protein MOP6 Neuronal PAS3 |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of NPAS3 in SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of NPAS3 in human brain tissue with NPAS3 antibody at 20 μg/mL.
Application
Immunohistochemistry of NPAS3 in human brain tissue with NPAS3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | NPAS3 antibody can be used for detection of NPAS3 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NPAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NPAS3 Antibody is supplied in PBS containing 0.02% sodium azide. |
