Application
Western blot analysis of SMMC - 7721 cell extracts with NPM1 antibody.Application
Immunohistochemical staining of paraffin embedded human liver carcinoma showing nuclear localization NPM1 atnibody with DAB staining.| Product Name | NPM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPM, Nucleolar phosphoprotein B23, NPM, B23 |
| Product description | Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM1 has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase .1) Morris SW, et al. Science 1994 Mar 4; 263(5151):1281-4. |
| Immunogen | Ni-NTA purified recombinant human NPM1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPM1 |
|---|---|
| Alternative Names | NPM Nucleolar phosphoprotein B23 NPM B23 |
Application
Western blot analysis of SMMC - 7721 cell extracts with NPM1 antibody.
Application
Immunohistochemical staining of paraffin embedded human liver carcinoma showing nuclear localization NPM1 atnibody with DAB staining.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NPM1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
