Application
Antibody used in IHC on Human Small Intestine at 5.0 ug/ml.Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.| Product Name | NR1I2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR1I2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR |
| Product description | NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. NR1I2 contains a zinc finger domain.NR1I2 is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.1) Hesselink, D.A., (2008) Pharmacogenomics 9 (6), 783-789. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR1I2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR1I2 antibody can be used for detection of NR1I2 by ELISA at 1:1562500. NR1I2 antibody can be used for detection of NR1I2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1I2 |
|---|---|
| Alternative Names | NR1I2 BXR ONR1 PAR PAR1 PAR2 PARq PRR PXR SAR SXR |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in IHC on Human Small Intestine at 5.0 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | NR1I2 antibody can be used for detection of NR1I2 by ELISA at 1:1562500. NR1I2 antibody can be used for detection of NR1I2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR1I2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
