Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Product Name | NR1I2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR1I2, BXR, PAR, PRR, PXR, SAR, SXR, ONR1, PAR1, PAR2, PARq |
| Product description | NR1I2 belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. NR1I2 contains a zinc finger domain.NR1I2 is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. The gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. The gene product contains a zinc finger domain. Three alternatively spliced transcripts that encode different isoforms have been described, one of which encodes two products through the use of alternative translation initiation codons. Additional transcript variants derived from alternative promoter usage, alternative splicing, and/or alternative polyadenylation exist, but they have not been fully described.1) Dring, M.M., (2006) Gastroenterology 130 (2), 341-348. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR1I2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR1I2 antibody can be used for detection of NR1I2 by ELISA at 1:12500. NR1I2 antibody can be used for detection of NR1I2 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR1I2 |
|---|---|
| Alternative Names | NR1I2 BXR PAR PRR PXR SAR SXR ONR1 PAR1 PAR2 PARq |
| Molecular Weight(MW) | 54 kDa, 50 kDa, 46 kDa |
Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NR1I2 antibody can be used for detection of NR1I2 by ELISA at 1:12500. NR1I2 antibody can be used for detection of NR1I2 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR1I2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
