NR5A1 is a members of the orphan nuclear receptor superfamily that is critical regulatory components of the hypothalamic-pituitary-adrenal-gonadal axis. In adrenal and gonadal tissues they regulate the expression of the cytochrome P450 steroid hydroxylase genes, key mediators of steroidogenesis.1) Li, L.A., et al., (2004) J. Steroid Biochem. Mol. Biol. 91 (1-2), 11--20.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR5A1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NR5A1 antibody can be used for detection of NR5A1 by ELISA at 1:62500. NR5A1 antibody can be used for detection of NR5A1 by western blot at 20 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
NR5A1 antibody can be used for detection of NR5A1 by ELISA at 1:62500. NR5A1 antibody can be used for detection of NR5A1 by western blot at 20 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR5A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt