Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
NR5A1 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot in HepG2 cell line lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.| Product Name | NR5A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Steroidogenic factor 1, SF-1, STF-1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP, NR5A1, AD4BP, FTZF1, SF1 |
| Product description | NR5A1 is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this protein are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.1) Almeida, M.Q., et al. J. Clin. Endocrinol. Metab. 95(3):1458-1462(2010) |
| Immunogen | This NR5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the N-terminal region of human NR5A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR5A1 |
|---|---|
| Alternative Names | Steroidogenic factor 1 SF-1 STF-1 Adrenal 4-binding protein Fushi tarazu factor homolog 1 Nuclear receptor subfamily 5 group A member 1 Steroid hormone receptor Ad4BP NR5A1 AD4BP FTZF1 SF1 |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
NR5A1 Antibody IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot in HepG2 cell line lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
