Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | NRCAM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NRCAM, KIAA0343, MGC138845, MGC138846 |
| Product description | Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. NRCAM is a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM may also play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of its gene have been associated with autism and addiction vulnerability.Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Ishiguro, H., (er) Neuropsychopharmacology (2005) In press. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NRCAM. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| NRCAM antibody can be used for detection of NRCAM by ELISA at 1:62500. NRCAM antibody can be used for detection of NRCAM by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NRCAM |
|---|---|
| Alternative Names | NRCAM KIAA0343 MGC138845 MGC138846 |
| Molecular Weight(MW) | 141 kDa, 131 kDa |
| Entrez Gene | 4897 |
|---|---|
| Protein Accession | NP_001032209 |
Application
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NRCAM antibody can be used for detection of NRCAM by ELISA at 1:62500. NRCAM antibody can be used for detection of NRCAM by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NRCAM antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
