Application
Antibody used in WB on Human Hela.Application
Antibody used in WB on Human 293T.Application
Antibody used in WB on Human Jurkat.Application
Antibody used in IHC on Human Prostate.Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | NRF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NRF1, ALPHA-PAL |
| Product description | This protein homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for 'nuclear factor (erythroid-derived 2)-like 1' which has an official symbol of NFE2L1.1) Ramachandran, B., (2008) J. Biol. Chem. 283 (18), 11935-11946. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NRF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NRF1 antibody can be used for detection of NRF1 by ELISA at 1:62500. NRF1 antibody can be used for detection of NRF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NRF1 |
|---|---|
| Alternative Names | NRF1 ALPHA-PAL |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human Hela.
Application
Antibody used in WB on Human 293T.
Application
Antibody used in WB on Human Jurkat.
Application
Antibody used in IHC on Human Prostate.
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | NRF1 antibody can be used for detection of NRF1 by ELISA at 1:62500. NRF1 antibody can be used for detection of NRF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NRF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
