Application
Western blot analysis in A549 cell line lysates (35ug/lane).Application
Fluorescent confocal image of Hela cell stained with NRK Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7| Product Name | NRK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nik-related protein kinase, NRK |
| Product description | The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.1) Ross, M.T., et al. Nature 434(7031):325-337(2005) |
| Immunogen | This NRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the Central region of human NRK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NRK |
|---|---|
| Alternative Names | Nik-related protein kinase NRK |
| Molecular Weight(MW) | 178 kDa |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
Fluorescent confocal image of Hela cell stained with NRK Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
