Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Product Name | NSDHL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NSDHL, H105E3, XAP104, SDR31E1 |
| Product description | NSDHL is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in NSDHL gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males.The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.1) Guggenberger, C., J. Steroid Biochem. Mol. Biol. 104 (3-5), 105-109 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSDHL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NSDHL antibody can be used for detection of NSDHL by ELISA at 1:312500. NSDHL antibody can be used for detection of NSDHL by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NSDHL |
|---|---|
| Alternative Names | NSDHL H105E3 XAP104 SDR31E1 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NSDHL antibody can be used for detection of NSDHL by ELISA at 1:312500. NSDHL antibody can be used for detection of NSDHL by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NSDHL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
