Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | NSUN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NSUN2, FLJ20303, MISU, SAKI, TRM4, MRT5 |
| Product description | Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).1) Huttenhofer, A., (2007) Mol. Biol. Cell 18 (3), 1107-1117. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NSUN2 |
|---|---|
| Alternative Names | NSUN2 FLJ20303 MISU SAKI TRM4 MRT5 |
| Molecular Weight(MW) | 86 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NSUN2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
