WB
Western blot analysis of NSUN2 on different lysates with Rabbit anti-NSUN2 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: HEK-293 cell lysate, Lane 5: 293T cell lysate, Lane 6: A431 cell lysate, Lane 7: HCT 116 cell lysate, Lane 8: Caco-2 cell lysate, Lane 9: Neuro-2a cell lysate, Lane 10: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-NSUN2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling NSUN2 with Rabbit anti-NSUN2 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN2 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.IP
NSUN2 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-NSUN2 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-NSUN2 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit anti-NSUN2 antibody IP in HeLa cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-NSUN2 antibody in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds.| Product Name | NSUN2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human NSUN2 aa1-767. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000 IHC:1:1000 ICC:1:250 IP:1-2μg/sample |
|
| Species Reactivity | HumanMonkeyMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NSUN2 |
|---|---|
| Gene Synonyms | MISU MRT5 SAKI TRM4 |
| Gene Full Name | NOP2/Sun RNA methyltransferase 2 |
| Gene Summary | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011] |
| Alternative Names | 5 methycytoisine methyltransferase antibody D13Wsu123e antibody FLJ20303 antibody hTrm4 antibody MISU antibody Myc induced SUN domain containing protein antibody NOL1/NOP2/Sun domain family 2 antibody NOL1/NOP2/Sun domain family 2 protein antibody NOL1/NOP2/Sun domain family member 2 antibody NSUN 2 antibody 5 methycytoisine methyltransferase antibody D13Wsu123e antibody FLJ20303 antibody hTrm4 antibody MISU antibody Myc induced SUN domain containing protein antibody NOL1/NOP2/Sun domain family 2 antibody NOL1/NOP2/Sun domain family 2 protein antibody NOL1/NOP2/Sun domain family member 2 antibody NSUN 2 antibody NSUN2 antibody NSUN2_HUMAN antibody SAKI antibody Substrate of AIM1/Aurora kinase B antibody TRM4 antibody tRNA (cytosine 5 ) methyltransferase antibody tRNA (cytosine 5 ) methyltransferase NSUN2 antibody tRNA (cytosine(34)-C(5))-methyltransferase antibody tRNA (cytosine-5-)-methyltransferase antibody tRNA methyltransferase 4 homolo antibody tRNA methyltransferase 4 homolog antibody |
| Molecular Weight(MW) | 87kDa(Observed band size: 100kDa) |
| Cellular Localization | Nucleus, nucleolus, Cytoplasm, Mitochondrion, cytoskeleton, spindle, Secreted, extracellular exosome. |
WB
Western blot analysis of NSUN2 on different lysates with Rabbit anti-NSUN2 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: HEK-293 cell lysate, Lane 5: 293T cell lysate, Lane 6: A431 cell lysate, Lane 7: HCT 116 cell lysate, Lane 8: Caco-2 cell lysate, Lane 9: Neuro-2a cell lysate, Lane 10: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-NSUN2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling NSUN2 with Rabbit anti-NSUN2 antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NSUN2 antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IP
NSUN2 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-NSUN2 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-NSUN2 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit anti-NSUN2 antibody IP in HeLa cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-NSUN2 antibody in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds.| Application Notes | WB:1:2000 IHC:1:1000 ICC:1:250 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
