Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | NT5C3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NT5C3, MGC27337, MGC87109, MGC87828, P5'N-1, PN-I, PSN1, UMPH, UMPH1, cN-III, p36, POMP, NT5C3, P5N-1, hUMP1 |
| Product description | Pyrimidine 5-prime-nucleotidase (P5N), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology. Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2; MIM 191720). The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.1) Chondrogianni, N. (2007) Exp. Gerontol. 42 (9), 899-903. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NT5C3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NT5C3 antibody can be used for detection of NT5C3 by ELISA at 1:1562500. NT5C3 antibody can be used for detection of NT5C3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NT5C3A |
|---|---|
| Alternative Names | NT5C3 MGC27337 MGC87109 MGC87828 P5'N-1 PN-I PSN1 UMPH UMPH1 cN-III p36 POMP NT5C3 P5N-1 hUMP1 |
| Molecular Weight(MW) | 34 kDa |
| Entrez Gene | 51251 |
|---|---|
| Protein Accession | NP_001002009 |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NT5C3 antibody can be used for detection of NT5C3 by ELISA at 1:1562500. NT5C3 antibody can be used for detection of NT5C3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NT5C3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
