Application
Western blot analysis in T47D cell line lysates (35ug/lane).| Product Name | NTAN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein N-terminal asparagine amidohydrolase, 351-, Protein NH2-terminal asparagine amidohydrolase, PNAA, Protein NH2-terminal asparagine deamidase, PNAD, Protein N-terminal Asn amidase, Protein N-terminal asparagine amidase, Protein NTN-amidase, NTAN1 |
| Product description | Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N-terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position (By similarity).1) Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010) |
| Immunogen | This NTAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-230 amino acids from the C-terminal region of human NTAN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NTAN1 |
|---|---|
| Alternative Names | Protein N-terminal asparagine amidohydrolase 351- Protein NH2-terminal asparagine amidohydrolase PNAA Protein NH2-terminal asparagine deamidase PNAD Protein N-terminal Asn amidase Protein N-terminal asparagine amidase Protein NTN-amidase NTAN1 |
| Molecular Weight(MW) | 35 kDa |
Application
Western blot analysis in T47D cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
