Application
Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the NTHL1 antibody detected the NTHL1 protein (arrow).| Product Name | NTHL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endonuclease III-like protein 1 {ECO:0000255|HAMAP-Rule:MF_03183}, hNTH1, 322- {ECO:0000255|HAMAP-Rule:MF_03183}, 429918 {ECO:0000255|HAMAP-Rule:MF_03183}, Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase {ECO:0000255|HAMAP-Rule:MF_ |
| Product description | The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.1) Wang, W., et al. Nucleic Acids Res. (2010) In press : |
| Immunogen | This NTHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human NTHL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NTHL1 |
|---|---|
| Alternative Names | Endonuclease III-like protein 1 {ECO:0000255|HAMAP-Rule:MF_03183} hNTH1 322- {ECO:0000255|HAMAP-Rule:MF_03183} 429918 {ECO:0000255|HAMAP-Rule:MF_03183} Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase {ECO:0000255|HAMAP-Rule:MF_ |
| Molecular Weight(MW) | 34 kDa |
Application
Western Blot at 1:1000 dilution Lane 1: DU145 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in Hela,NCI-H460 cell line lysates (35ug/lane).This demonstrates the NTHL1 antibody detected the NTHL1 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
