Application
Antibody used in WB on Human HepG2 at: 1:1000 (Lane 1: 50ug HEK293 lysate, Lane 2: 50ug MDCK lysate, Lane 3: 50ug NMuMG lysate, Lane 4: 50ug MDAMB231 lysate).Application
Antibody used in IHC on Human Intestine.Application
Antibody used in WB on Human , canine, Mouse at: 1:1000 (Lane 1: 50ug HEK293 lysate, Lane 2: 50ug MDCK lysate, Lane 3: 50ug NMuMG lysate, Lane 4: 50ug MDAMB231 lysate).| Product Name | NUCB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUCB2, NEFA, HEL-S-109 |
| Product description | Nucleobindin-2 is a calcium-binding EF-hand protein.1) Line, A., et al., (2002) J. Cancer 86 (11), 1824-1830. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUCB2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| NUCB2 antibody can be used for detection of NUCB2 by ELISA at 1:312500. NUCB2 antibody can be used for detection of NUCB2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NUCB2 |
|---|---|
| Alternative Names | NUCB2 NEFA HEL-S-109 |
| Molecular Weight(MW) | 46 kDa |
Application
Antibody used in WB on Human HepG2 at: 1:1000 (Lane 1: 50ug HEK293 lysate, Lane 2: 50ug MDCK lysate, Lane 3: 50ug NMuMG lysate, Lane 4: 50ug MDAMB231 lysate).
Application
Antibody used in IHC on Human Intestine.
Application
Antibody used in WB on Human , canine, Mouse at: 1:1000 (Lane 1: 50ug HEK293 lysate, Lane 2: 50ug MDCK lysate, Lane 3: 50ug NMuMG lysate, Lane 4: 50ug MDAMB231 lysate).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NUCB2 antibody can be used for detection of NUCB2 by ELISA at 1:312500. NUCB2 antibody can be used for detection of NUCB2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NUCB2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
