Application
Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.Application
Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.Application
Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | Nucleobindin-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nucleobindin-2 Antibody: NEFA, HEL-S-109, NEFA, Nucleobindin-2, DNA-binding protein NEFA |
| Product description | Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.1) Taniguchi N, Taniura H, Niinobe M, et al. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 2000; 275:31674-85. |
| Immunogen | Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 . The immunogen is located within amino acids 260 - 310 of Nucleobindin-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUCB2 |
|---|---|
| Alternative Names | Nucleobindin-2 Antibody: NEFA HEL-S-109 NEFA Nucleobindin-2 DNA-binding protein NEFA |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | Nucleobindin-2 antibody is predicted to not cross-react with NUCB1. |
Application
Immunofluorescence of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 20 μg/ml.
Application
Immunohistochemistry of Nucleobindin-2 in rat brain tissue with Nucleobindin-2 antibody at 2.5 μg/ml.
Application
Western blot analysis of Nucleobindin-2 in rat brain tissue lysate with Nucleobindin-2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Nucleobindin-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
