Application
Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 μg/mL.Application
Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 μg/mL.Application
Western blot analysis of Nudel in Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 μg/mL.| Product Name | Nudel Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nudel Antibody: EOPA, NDE2, NUDEL, MITAP1, NDE1L1, EOPA, Nuclear distribution protein nudE-like 1, Mitosin-associated protein 1, Protein Nudel |
| Product description | Nudel Antibody: Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.1) Niethammer H, Smith DS, Ayala R, et al. NUDEL is a novel Cdk5 substrate that associates with LIS1 and cytoplasmic dynein. Neuron 2000; 28:697-711. |
| Immunogen | Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel. The immunogen is located within the first 50 amino acids of Nudel. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDEL1 |
|---|---|
| Alternative Names | Nudel Antibody: EOPA NDE2 NUDEL MITAP1 NDE1L1 EOPA Nuclear distribution protein nudE-like 1 Mitosin-associated protein 1 Protein Nudel |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of Nudel in Jurkat cells with Nudel antibody at 2 μg/mL.
Application
Immunofluorescence of Nudel in Jurkat cells with Nudel antibody at 10 μg/mL.
Application
Western blot analysis of Nudel in Jurkat whole cell lysate with Nudel antibody at (A) 0.5, (B) 1, or (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Nudel antibody can be used for detection of Nudel by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Nudel antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nudel Antibody is supplied in PBS containing 0.02% sodium azide. |
