Application
NUDT15 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + HuTu80 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:1000 dilution + SW620 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NUDT15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable 8-oxo-dGTP diphosphatase NUDT15, 8-oxo-dGTPase NUDT15,8-dihydro-8-oxoguanine-triphosphatase NUDT15, MutT homolog 2, MTH2, Nucleoside diphosphate-linked moiety X motif 15, Nudix motif 15, NUDT15, MTH2 |
| Product description | Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear (By similarity). May have a role in DNA synthesis and cell cycle progression throught the interaction with PCNA.1) Hori, M., et al. Free Radic. Biol. Med. 48(9):1197-1201(2010) Yu, Y., et al. J. Biol. Chem. 284(29):19310-19320(2009) Dunham, A., et al. Nature 428(6982):522-528(2004) Cai, J.P., et al. Biochem. Biophys. Res. Commun. 305(4):1073-1077(2003) |
| Immunogen | This NUDT15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the C-terminal region of human NUDT15. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUDT15 |
|---|---|
| Alternative Names | Probable 8-oxo-dGTP diphosphatase NUDT15 8-oxo-dGTPase NUDT15 8-dihydro-8-oxoguanine-triphosphatase NUDT15 MutT homolog 2 MTH2 Nucleoside diphosphate-linked moiety X motif 15 Nudix motif 15 NUDT15 MTH2 |
| Molecular Weight(MW) | 19 kDa |
Application
NUDT15 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + HuTu80 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:1000 dilution + SW620 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
