Application
Immunohistochemistry of NUMB in human lung tissue with NUMB antibody at 5 μg/mL.Application
Immunofluorescence of NUMB in human lung tissue with NUMB antibody at 20 μg/mL.Application
Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 μg/mL.| Product Name | NUMB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUMB Antibody: S171, C14orf41, c14_5527, Protein numb homolog, Protein S171, h-Numb |
| Product description | NUMB Antibody: NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.1) Zhong W, Feder JN, Jiang MM, et al. Asymmetric localization of a mammalian numb homolog during mouse cortical neurogenesis. Neuron 1996; 17:43-53. |
| Immunogen | NUMB antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human NUMB. The immunogen is located within the last 50 amino acids of NUMB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NUMB antibody can be used for detection of NUMB by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUMB |
|---|---|
| Alternative Names | NUMB Antibody: S171 C14orf41 c14_5527 Protein numb homolog Protein S171 h-Numb |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least four isoforms of NUMB are known to exist; this antibody will detect all of them. |
| Entrez Gene | 8650 |
|---|---|
| Protein Accession | NP_1005743 |
Application
Immunohistochemistry of NUMB in human lung tissue with NUMB antibody at 5 μg/mL.
Application
Immunofluorescence of NUMB in human lung tissue with NUMB antibody at 20 μg/mL.
Application
Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | NUMB antibody can be used for detection of NUMB by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NUMB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NUMB Antibody is supplied in PBS containing 0.02% sodium azide. |
