Application
Immunocytochemistry of NUP107 in A549 cells with NUP107 antibody at 10 μg/mL.Application
Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 μg/mL.Application
Western blot analysis of NUP107 in A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NUP107 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUP107 Antibody: NUP84, Nuclear pore complex protein Nup107, 107 kDa nucleoporin |
| Product description | NUP107 Antibody: The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.1) Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell2006; 125:1041-53. |
| Immunogen | NUP107 antibody was raised against a 20 amino acid synthetic peptide from near the center of human NUP107. The immunogen is located within amino acids 370 - 420 of NUP107. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| NUP107 antibody can be used for detection of NUP107 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP107 |
|---|---|
| Alternative Names | NUP107 Antibody: NUP84 Nuclear pore complex protein Nup107 107 kDa nucleoporin |
| Sequence Similarities | 2) Cat. No. 17-003 - A549 Cell Slide |
Application
Immunocytochemistry of NUP107 in A549 cells with NUP107 antibody at 10 μg/mL.
Application
Immunofluorescence of NUP107 in A549 cells with NUP107 antibody at 10 μg/mL.
Application
Western blot analysis of NUP107 in A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | NUP107 antibody can be used for detection of NUP107 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NUP107 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NUP107 Antibody is supplied in PBS containing 0.02% sodium azide. |
