Application
Immunohistochemistry of NUP160 in human brain tissue with NUP160 antibody at 2.5 μg/mL.Application
Western blot analysis of NUP160 in rat brain tissue lysate with NUP160 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of NUP160 in Human Brain cells with NUP160 antibody at 20 μg/mL.| Product Name | NUP160 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUP160 Antibody: KIAA0197, NUP120, Nuclear pore complex protein Nup160, 160 kDa nucleoporin |
| Product description | NUP160 Antibody: The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-160 is part of the hetero-oligomeric complex that also contains NUP107, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP160 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP160 may be an important drug target in HIV treatment. Multiple isoforms of NUP160 are known to exist.1) Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell2006; 125:1041-53. |
| Immunogen | NUP160 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human NUP160. The immunogen is located within the last 50 amino acids of NUP160. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NUP160 antibody can be used for detection of NUP160 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP160 |
|---|---|
| Alternative Names | NUP160 Antibody: KIAA0197 NUP120 Nuclear pore complex protein Nup160 160 kDa nucleoporin |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of NUP160 in human brain tissue with NUP160 antibody at 2.5 μg/mL.
Application
Western blot analysis of NUP160 in rat brain tissue lysate with NUP160 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of NUP160 in Human Brain cells with NUP160 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | NUP160 antibody can be used for detection of NUP160 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NUP160 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NUP160 Antibody is supplied in PBS containing 0.02% sodium azide. |
