The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. NUP54 is a member of the phe-gly (FG) repeat-containing nucleoporin subset.1) Stoffler, D., et al., Curr. Opin. Cell Biol. 11(3):391-401 (1999).
Immunogen
This NUP54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-243 amino acids from the Central region of human NUP54.
Western blot analysis of anti-NUP54 Pab in mouse heart tissue lysate.
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.