Application
Flow cytometric analysis of MDA-231 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of in HepG2 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | NUP62 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear pore glycoprotein p62, 62 kDa nucleoporin, Nucleoporin Nup62, NUP62 |
| Product description | NUP62 is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This protein is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. The protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals.1) Stochaj,U., Banski,P. Exp. Cell Res. 312 (13), 2490-2499 (2006) |
| Immunogen | This NUP62 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from the C-terminal region of human NUP62. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NUP62 |
|---|---|
| Alternative Names | Nuclear pore glycoprotein p62 62 kDa nucleoporin Nucleoporin Nup62 NUP62 |
| Molecular Weight(MW) | 53 kDa |
Application
Flow cytometric analysis of MDA-231 cells using (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of in HepG2 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
