Application
Immunofluorescence of OASL1 in HepG2 cells with OASL1 antibody at 20 μg/mL.Application
Immunocytochemistry of OASL1 in HepG2 cells with OASL1 antibody at 2.5 μg/mL.Application
Western blot analysis of OASL in mouse bladder tissue lysate with OASL antibody at 1 μg/ml.| Product Name | OASL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OASL Antibody: OASLd, TRIP14, TRIP-14, p59OASL, p59 OASL, p59-OASL, Thyroid receptor-interacting protein 14, TR-interacting protein 14 |
| Product description | OASL (2'-5'-oligoadenylate synthetase-like), also known as p59OASL or TRIP14 (thyroid receptor-interacting protein 14), is a 514 amino acid protein that exists as two alternatively spliced isoforms, designated p56 and p30, and contains two ubiquitin-like domains (1). It is widely expressed in a variety of tissues and interacts with the ligand binding domain of the thyroid receptor (TR) and is able to bind double-stranded RNA and DNA, possibly playing a role in RNA degradation and the overall inhibition of protein synthesis (2-3). Methyl CpG-binding protein 1 (MBD1), which functions as a transcriptional repressor, was identified as a strong p59 OASL interactor (4).1) Rebouillat D, Marie I, and Hovanessian AG. Molecular cloning and characterization of two related and interferon-induced 56 kDa and 30 kDa proteins highly similar to 2'-5' oligoadenylate synthetase. Eur. J. Biochem. 1998; 257:319-30. |
| Immunogen | OASL antibody was raised against a 17 amino acid peptide near the amino terminus of human OASL. The immunogen is located within the first 50 amino acids of OASL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| OASL antibody can be used for detection of OASL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OASL |
|---|---|
| Alternative Names | OASL Antibody: OASLd TRIP14 TRIP-14 p59OASL p59 OASL p59-OASL Thyroid receptor-interacting protein 14 TR-interacting protein 14 |
| Molecular Weight(MW) | Predicted: 57 kDa Observed: 65 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Tissue Specificity | OASL antibody is human, mouse and rat reactive. |
Application
Immunofluorescence of OASL1 in HepG2 cells with OASL1 antibody at 20 μg/mL.
Application
Immunocytochemistry of OASL1 in HepG2 cells with OASL1 antibody at 2.5 μg/mL.
Application
Western blot analysis of OASL in mouse bladder tissue lysate with OASL antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1410 - Mouse Bladder Tissue Lysate |
|---|---|
| Application Notes | OASL antibody can be used for detection of OASL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OASL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | OASL Antibody is supplied in PBS containing 0.02% sodium azide. |
