Application
Immunofluorescence of OCIAD1 in 293 cells with OCIAD1 antibody at 20 μg/mL.Application
Western blot analysis of OCIAD1 in 293 cell lysate with OCIAD1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | OCIAD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OCIAD1 Antibody: OCIA, ASRIJ, TPA018, OCIA, OCIA domain-containing protein 1, Ovarian carcinoma immunoreactive antigen |
| Product description | OCIAD1 Antibody: OCIAD1 was identified via immunoscreening of an ovarian carcinoma cDNA library from ovarian cancer patients and is expressed in multiple tissues including ovary, placenta, brain, testis, prostate, and mammary gland. Two isoforms of OCIAD1 are known to exist; the shorter isoform is restricted to the central nervous system. OCIAD1 is a transmembrane protein whose overexpression in HEY ovarian cancer cells increased lysophosphatidic acid- (LPA-)induced, but not basal level cell adhesion to extracellular matrix proteins collagen I and laminin10/11. This adhesion is not blocked by LY294002 and GF109203X, suggesting that OCIAD1 does not use protein kinase C and PI3 kinase signaling pathways to exert its effect on adhesion.1) Luo LY, Soosaipillai A, and Diamandis EP. Molecular cloning of a novel human gene on chromosome 4p11 by immunoscreening of an ovarian carcinoma cDNA library. Biochem. Biophys. Res. Commun.2001; 280:401-6. |
| Immunogen | OCIAD1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human OCIAD1. The immunogen is located within the last 50 amino acids of OCIAD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| OCIAD1 antibody can be used for detection of OCIAD1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OCIAD1 |
|---|---|
| Alternative Names | OCIAD1 Antibody: OCIA ASRIJ TPA018 OCIA OCIA domain-containing protein 1 Ovarian carcinoma immunoreactive antigen |
| Sequence Similarities | 2) Cat. No. 17-010 - HEK293 Cell Slide |
Application
Immunofluorescence of OCIAD1 in 293 cells with OCIAD1 antibody at 20 μg/mL.
Application
Western blot analysis of OCIAD1 in 293 cell lysate with OCIAD1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | OCIAD1 antibody can be used for detection of OCIAD1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OCIAD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OCIAD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
